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dna spotting solution  (CapitalBio Corporation)

 
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    CapitalBio Corporation dna spotting solution
    ( A ) Schematic of the i-chip (left). The i-chip consisted of a 3D-printed sample preparation unit and a microfluidic amplification unit. The sample preparation unit was designed to perform the entire process of POC <t>DNA</t> pretreatment using a basket module, a rotary valve, and a block module. The basket module had a DNA extraction disc at its bottom (dotted box). ( B ) Schematic of the microfluidic amplification unit. The microfluidic amplification unit consisted of a microfluidic channel layer and <t>an</t> <t>aldehyde-functionalized</t> glass chip. The probe channel layer (marked in red) was used to modify the capture probes on the aldehyde-functionalized chip. The microfluidic channel layer (marked in pink) was then assembled onto the chip to complete the amplification unit. The cross section of the probe fluidic and microfluidic channels constituted the reaction zone (dotted box). The numbers 1, 2, 3, and 4 on the probe channel layer indicated different probe channels with different colors. ( C ) Detection procedure of POCKET system. DNA was absorbed and extracted using the extraction disc at the bottom of the basket. Target DNA was purified using a wash buffer and amplified by RPA to obtain biotin-modified amplicons. The amplicons were bound to SA-AuNPs and captured by premodified capture probes to achieve a secondary signal amplification. The tertiary amplification was initiated by depositing silver ions onto the AuNPs. ( D ) The POCKET platform was scalable for parallel sample detections by i-chips assembly. ( E ) Schematic of the f-box, which was designed to display the results. This included a foldable cradle, a smartphone, an optical unit with a macro-lens, and a LED lamp. Photo credit (D): Huan Xu, Army Medical University.
    Dna Spotting Solution, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+spotting+solution/dna+spotting+buffer/pmc07176422-202-9-12
    Average 90 stars, based on 1 article reviews
    dna spotting solution - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "An ultraportable and versatile point-of-care DNA testing platform"

    Article Title: An ultraportable and versatile point-of-care DNA testing platform

    Journal: Science Advances

    doi: 10.1126/sciadv.aaz7445

    ( A ) Schematic of the i-chip (left). The i-chip consisted of a 3D-printed sample preparation unit and a microfluidic amplification unit. The sample preparation unit was designed to perform the entire process of POC DNA pretreatment using a basket module, a rotary valve, and a block module. The basket module had a DNA extraction disc at its bottom (dotted box). ( B ) Schematic of the microfluidic amplification unit. The microfluidic amplification unit consisted of a microfluidic channel layer and an aldehyde-functionalized glass chip. The probe channel layer (marked in red) was used to modify the capture probes on the aldehyde-functionalized chip. The microfluidic channel layer (marked in pink) was then assembled onto the chip to complete the amplification unit. The cross section of the probe fluidic and microfluidic channels constituted the reaction zone (dotted box). The numbers 1, 2, 3, and 4 on the probe channel layer indicated different probe channels with different colors. ( C ) Detection procedure of POCKET system. DNA was absorbed and extracted using the extraction disc at the bottom of the basket. Target DNA was purified using a wash buffer and amplified by RPA to obtain biotin-modified amplicons. The amplicons were bound to SA-AuNPs and captured by premodified capture probes to achieve a secondary signal amplification. The tertiary amplification was initiated by depositing silver ions onto the AuNPs. ( D ) The POCKET platform was scalable for parallel sample detections by i-chips assembly. ( E ) Schematic of the f-box, which was designed to display the results. This included a foldable cradle, a smartphone, an optical unit with a macro-lens, and a LED lamp. Photo credit (D): Huan Xu, Army Medical University.
    Figure Legend Snippet: ( A ) Schematic of the i-chip (left). The i-chip consisted of a 3D-printed sample preparation unit and a microfluidic amplification unit. The sample preparation unit was designed to perform the entire process of POC DNA pretreatment using a basket module, a rotary valve, and a block module. The basket module had a DNA extraction disc at its bottom (dotted box). ( B ) Schematic of the microfluidic amplification unit. The microfluidic amplification unit consisted of a microfluidic channel layer and an aldehyde-functionalized glass chip. The probe channel layer (marked in red) was used to modify the capture probes on the aldehyde-functionalized chip. The microfluidic channel layer (marked in pink) was then assembled onto the chip to complete the amplification unit. The cross section of the probe fluidic and microfluidic channels constituted the reaction zone (dotted box). The numbers 1, 2, 3, and 4 on the probe channel layer indicated different probe channels with different colors. ( C ) Detection procedure of POCKET system. DNA was absorbed and extracted using the extraction disc at the bottom of the basket. Target DNA was purified using a wash buffer and amplified by RPA to obtain biotin-modified amplicons. The amplicons were bound to SA-AuNPs and captured by premodified capture probes to achieve a secondary signal amplification. The tertiary amplification was initiated by depositing silver ions onto the AuNPs. ( D ) The POCKET platform was scalable for parallel sample detections by i-chips assembly. ( E ) Schematic of the f-box, which was designed to display the results. This included a foldable cradle, a smartphone, an optical unit with a macro-lens, and a LED lamp. Photo credit (D): Huan Xu, Army Medical University.

    Techniques Used: Sample Prep, Amplification, Blocking Assay, DNA Extraction, Purification, Modification

    Related Articles

    Concentration Assay:

    Article Title: DNA Microarray Detection of 18 Important Human Blood Protozoan Species
    Article Snippet: .. Oligonucleotide probes were contact printed onto OPAldehyde Slide aldehyde-activated slides at a concentration of 10μM in DNA spotting solution on a Smart Arrayer-96 Microarrayer (both from CapitalBio, Beijing, China), and covalently immobilized on the slides via an amino group at their 5’ ends [ – ] to create biochips ( ). .. All oligonucleotide primers and probes listed in and Tables were obtained from CapitalBio Corporation, Beijing, China.

    Article Title: Multiplex Asymmetric PCR-Based Oligonucleotide Microarray for Detection of Drug Resistance Genes Containing Single Mutations in Enterobacteriaceae
    Article Snippet: Microarrays were processed and spotted using a SmartArray-48 microarrayer (CapitalBio Co., Ltd., Beijing, People's Republic of China). .. The oligonucleotide probes were applied to the aldehyde-activated slides (CapitalBio) at a concentration of 10 μM in DNA Spotting solution (CapitalBio) and covalently immobilized on the slides by the mediation of an amino group at their 5′ ends. ..

    Article Title: Identification of novel stress-regulated microRNAs from Oryza sativa L.
    Article Snippet: .. Oligonucleotide probes were synthesized by Invitrogen (Shanghai, China) and dissolved in DNA Spotting Solution (CapitalBio Corp., Beijing, China) at a concentration of 40 mM and printed on the aldehyde-modified glass slides (CapitalBio) in triplicate using a SmartArrayTM-136 microarrayer (CapitalBio). ..

    Polymerase Chain Reaction:

    Article Title: Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model.
    Article Snippet: .. The confirmed PCR products of 152 genes were precipitated with 2-propanol and redissolved in DNA Spotting Solution (CapitalBio Corp., Beijing, China). ..

    Article Title: Comparative and functional genomics reveals genetic diversity and determinants of host specificity among reference strains and a large collection of Chinese isolates of the phytopathogen Xanthomonas campestris pv. campestris
    Article Snippet: .. The confirmed PCR products were precipitated with isopropanol and redissolved in DNA Spotting Solution (CapitalBio Corp., Beijing, China). ..

    Synthesized:

    Article Title: Identification of novel stress-regulated microRNAs from Oryza sativa L.
    Article Snippet: .. Oligonucleotide probes were synthesized by Invitrogen (Shanghai, China) and dissolved in DNA Spotting Solution (CapitalBio Corp., Beijing, China) at a concentration of 40 mM and printed on the aldehyde-modified glass slides (CapitalBio) in triplicate using a SmartArrayTM-136 microarrayer (CapitalBio). ..

    Injection:

    Article Title: An ultraportable and versatile point-of-care DNA testing platform
    Article Snippet: .. Then, 5 μM amino-functionalized DNA probes (Tsingke, China) in DNA spotting solution (CapitalBio, China) were then injected into each channel and incubated for 2 hours at room temperature to modify the probes on the surface of glass chip through aldimine condensation. ..

    Incubation:

    Article Title: An ultraportable and versatile point-of-care DNA testing platform
    Article Snippet: .. Then, 5 μM amino-functionalized DNA probes (Tsingke, China) in DNA spotting solution (CapitalBio, China) were then injected into each channel and incubated for 2 hours at room temperature to modify the probes on the surface of glass chip through aldimine condensation. ..



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    ( A ) Schematic of the i-chip (left). The i-chip consisted of a 3D-printed sample preparation unit and a microfluidic amplification unit. The sample preparation unit was designed to perform the entire process of POC <t>DNA</t> pretreatment using a basket module, a rotary valve, and a block module. The basket module had a DNA extraction disc at its bottom (dotted box). ( B ) Schematic of the microfluidic amplification unit. The microfluidic amplification unit consisted of a microfluidic channel layer and <t>an</t> <t>aldehyde-functionalized</t> glass chip. The probe channel layer (marked in red) was used to modify the capture probes on the aldehyde-functionalized chip. The microfluidic channel layer (marked in pink) was then assembled onto the chip to complete the amplification unit. The cross section of the probe fluidic and microfluidic channels constituted the reaction zone (dotted box). The numbers 1, 2, 3, and 4 on the probe channel layer indicated different probe channels with different colors. ( C ) Detection procedure of POCKET system. DNA was absorbed and extracted using the extraction disc at the bottom of the basket. Target DNA was purified using a wash buffer and amplified by RPA to obtain biotin-modified amplicons. The amplicons were bound to SA-AuNPs and captured by premodified capture probes to achieve a secondary signal amplification. The tertiary amplification was initiated by depositing silver ions onto the AuNPs. ( D ) The POCKET platform was scalable for parallel sample detections by i-chips assembly. ( E ) Schematic of the f-box, which was designed to display the results. This included a foldable cradle, a smartphone, an optical unit with a macro-lens, and a LED lamp. Photo credit (D): Huan Xu, Army Medical University.
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    Image Search Results


    ( A ) Schematic of the i-chip (left). The i-chip consisted of a 3D-printed sample preparation unit and a microfluidic amplification unit. The sample preparation unit was designed to perform the entire process of POC DNA pretreatment using a basket module, a rotary valve, and a block module. The basket module had a DNA extraction disc at its bottom (dotted box). ( B ) Schematic of the microfluidic amplification unit. The microfluidic amplification unit consisted of a microfluidic channel layer and an aldehyde-functionalized glass chip. The probe channel layer (marked in red) was used to modify the capture probes on the aldehyde-functionalized chip. The microfluidic channel layer (marked in pink) was then assembled onto the chip to complete the amplification unit. The cross section of the probe fluidic and microfluidic channels constituted the reaction zone (dotted box). The numbers 1, 2, 3, and 4 on the probe channel layer indicated different probe channels with different colors. ( C ) Detection procedure of POCKET system. DNA was absorbed and extracted using the extraction disc at the bottom of the basket. Target DNA was purified using a wash buffer and amplified by RPA to obtain biotin-modified amplicons. The amplicons were bound to SA-AuNPs and captured by premodified capture probes to achieve a secondary signal amplification. The tertiary amplification was initiated by depositing silver ions onto the AuNPs. ( D ) The POCKET platform was scalable for parallel sample detections by i-chips assembly. ( E ) Schematic of the f-box, which was designed to display the results. This included a foldable cradle, a smartphone, an optical unit with a macro-lens, and a LED lamp. Photo credit (D): Huan Xu, Army Medical University.

    Journal: Science Advances

    Article Title: An ultraportable and versatile point-of-care DNA testing platform

    doi: 10.1126/sciadv.aaz7445

    Figure Lengend Snippet: ( A ) Schematic of the i-chip (left). The i-chip consisted of a 3D-printed sample preparation unit and a microfluidic amplification unit. The sample preparation unit was designed to perform the entire process of POC DNA pretreatment using a basket module, a rotary valve, and a block module. The basket module had a DNA extraction disc at its bottom (dotted box). ( B ) Schematic of the microfluidic amplification unit. The microfluidic amplification unit consisted of a microfluidic channel layer and an aldehyde-functionalized glass chip. The probe channel layer (marked in red) was used to modify the capture probes on the aldehyde-functionalized chip. The microfluidic channel layer (marked in pink) was then assembled onto the chip to complete the amplification unit. The cross section of the probe fluidic and microfluidic channels constituted the reaction zone (dotted box). The numbers 1, 2, 3, and 4 on the probe channel layer indicated different probe channels with different colors. ( C ) Detection procedure of POCKET system. DNA was absorbed and extracted using the extraction disc at the bottom of the basket. Target DNA was purified using a wash buffer and amplified by RPA to obtain biotin-modified amplicons. The amplicons were bound to SA-AuNPs and captured by premodified capture probes to achieve a secondary signal amplification. The tertiary amplification was initiated by depositing silver ions onto the AuNPs. ( D ) The POCKET platform was scalable for parallel sample detections by i-chips assembly. ( E ) Schematic of the f-box, which was designed to display the results. This included a foldable cradle, a smartphone, an optical unit with a macro-lens, and a LED lamp. Photo credit (D): Huan Xu, Army Medical University.

    Article Snippet: Then, 5 μM amino-functionalized DNA probes (Tsingke, China) in DNA spotting solution (CapitalBio, China) were then injected into each channel and incubated for 2 hours at room temperature to modify the probes on the surface of glass chip through aldimine condensation.

    Techniques: Sample Prep, Amplification, Blocking Assay, DNA Extraction, Purification, Modification